Navigate Up
Sign In
home |  mail |  workspaces |  intranet |  contact 



 Dr. Ir. Leon van den Broeke

​Profile

Born on May 2nd 1962 in Veere, Leon van den Broeke obtained his MSc in Toxicology and Human Nutrition at the Wageningen University in 1988, and received his PhD degree in Pharmacochemistry on “Thiocompounds as potential UV radiation protectors” at the Leiden/Amsterdam Center for Drug Research in 1994. As a postdoctoral researcher, he worked at the Karolinska Institute and National Institute for Working Life in Stockholm (Sweden) on “Mechanistic studies on contact allergy to cobalt-, chromate- and nickel-salts”, from 1994-1998. For his second post-doc he joined the National Institutes of Health in Bethesda (USA), to study for 5 years “Enhancement of tumor-specific epitopes in relation to cancer vaccine development” and “The role of NK and NKT cells in tumor regression”. After a commercial side-step, between 2003-2009, at A. Menarini Diagnostics (Product Specialist) and Luminex BV (Customer Support Manager, and Field Marketing Scientist), he instructed chemistry courses at the Academy of Technology & Innovation and the Delta Academy of the HZ University of Applied Sciences in Vlissingen until 2015. Currently he is co-teaching a number of laboratory courses at the University College Roosevelt.

Publications

  • Mensink RP, de Groot MJM, Van den Broeke LT, Severijnen-Nobels AP, Demacker PNM and Katan MB: Effects of monounsaturated fatty acids v  complex carbohydrates on serum lipoproteins and apoproteins in healthy men and women. Metabolism 38: 172-178, 1989

  • Reed CJ, Van den Broeke LT and De Matteis F: Drug induced protoporphyria in the olfactory mucosa of the hamster. J Biochem Toxicol 4: 161-164, 1989

  • Beijersbergen van Henegouwen GMJ, de Vries H, Van den Broeke LT and Junginger HE: RRR-Tocopherols and their acetates as a possible scavenger of free radicals produced in the skin upon UVA-exposure. An  in vivo screening method. Fat Sci Technol 94: 24-27, 1992

  • Wierckx FCJ, Beijersbergen van Henegouwen GMJ, Van den Broeke LT, de Vries H, Meerman JHN and Mulder GJ: Photoactivation of 2-nitrofluorene  in vitro and in the rat in vivo. UVA-induced formation of reactive intermediates that bind covalently to RNA and protein. Carcinogenesis 13: 1759-1762, 1992

  • Van den Broeke LT and Beijersbergen van Henegouwen GMJ: Thiols as potential UV radiation protectors. An in vitro study. J Photochem Photobiol B: Biol 17: 279-286, 1993

  • Van den Broeke LT and Beijersbergen van Henegouwen GMJ: UV-radiation protecting efficacy of thiols, studied with UVA-induced binding of 8-MOP and CPZ to rat epidermal biomacromolecules in vivo. Int J Radiat Biol 63: 493-500, 1993

  • Van den Broeke LT, Ouijja EH, Bojarski J and Beijersbergen van Henegouwen GMJ: In vitro photodegradation of chlorpromazine. Photochem Photobiol  59: 140-144, 1994

  • Van den Broeke LT and Beijersbergen van Henegouwen GMJ: The effect of N-acetylcysteine on the UVB-induced inhibition of epidermal DNA synthesis in rat skin. J Photochem Photobiol B: Biol 26: 271-276, 1994

  • Van den Broeke LT and Beijersbergen van Henegouwen GMJ: Topically applied N-acetylcysteine as a protector against UVB-induced systemic immunosuppression. J Photochem Photobiol B: Biol 27: 61-65, 1995

  • Van den Broeke LT and Beijersbergen van Henegouwen GMJ: UV-radiation protecting efficacy of cysteine derivatives, studies with UVA-induced binding of 8-MOP and CPZ to rat epidermal biomacromolecules in vivo. Int J Radiat Biol 67: 411-420, 1995

  • Alert D, Gers-Barlag H, Van den Broeke LT and Beijersbergen van Henegouwen GMJ: Cosmetic and dermatological sunscreen compositions containing thiols and/or thiol derivatives. EP Patent Appl No 93919073.2, EP Patent No 0656774 B1, 22 Jan 1997

  • Degwert J, Gers-Barlag H, Van den Broeke LT and Beijersbergen van Henegouwen GMJ: Use of radical catchers as immunomodulating agents in cosmetic and dermatological compositions. EP Patent Appl No 93918914.8, EP Patent No 0656773B1, 2 Apr 1997

  • Alert D, Gers-Barlag H, Van den Broeke LT and Beijersbergen van Henegouwen GMJ: Cosmetic and dermatological sunscreen compositions containing thiols and/or thiol derivatives. US Patent Appl No 08/670295, US Patent No 5728373, 17 Mar 1998

  • Van den Broeke LT, Gräslund A, Nilsson JLG, Wahlberg JE, Scheynius A and Karlberg AT: Free radicals as potential mediators of metal-allergy: Ni2+ and Co2+ mediated free radical generation. Eur J Pharm Sci, 6: 279-286, 1998

  • Van den Broeke LT, Gräslund A, Larsson PH, Nilsson JLG, Wahlberg JE, Scheynius A and Karlberg AT: Free radicals as potential mediators of metal allergy: Effect of ascorbic acid on lymphocyte proliferation and IFN- production in contact allergy to Ni2+ and Co2+. Acta Derm Venereol (Stockh) 78: 95-98, 1998

  • Van den Broeke LT, Nilsson JLG, Wahlberg JE, Scheynius A and Karlberg AT: Lack of antagonism of Ni2+ and Co2+ allergy by other essential divalent metal ions. Contact Dermatitis 38: 266-273, 1998

  • Van den Broeke LT, Nilsson JLG, Scheynius A, Wahlberg JE and Karlberg A-T: Interactions of divalent ions in nickel-sensitive subjects (Letter to the Editor). Contact Dermatitis 39: 332, 1998

  • Van den Broeke LT, Heffler L, Nilsson JLG, Karlberg A-T and Scheynius A: Evidence for direct Ni2+ antigen formation on cultured human dendritic cells. Immunol 96: 578-585, 1999

  • Maher VE, Worley BS, Contois D, Smith MC, Kelley MJ, Stipanov M, Khleif SN, Goletz T, Van den Broeke LT, Mackall C, Helman LJ, Carbone DP and Berzofsky JA: Mutant oncogene and tumor suppressor gene products and fusion proteins created by chromosomal translocations as targets for cancer vaccines. In: WM Kast (ed.), Peptide-based cancer vaccines, Landes Bioscience, Austin, TX:  pp. 17-39, 2000

  • Worley BS, Van den Broeke LT, Goletz TJ, Pendleton CD, Daschbach EM, Thomas EK, Marincola FM, Helman LJ and Berzofsky JA: Antigenicity of fusion proteins from sarcoma-associated chromosomal translocations. Cancer Res 61: 6868-6875, 2001

  • Van den Broeke LT, Daschbach E, Thomas EK, Andringa G and Berzofsky JA: Dendritic cell induced activation of adaptive and innate anti-tumor immunity. J Immunol 171: 5842-5852, 2003

  • Van den Broeke LT: Biosensoren voor continue glucose- en melkzuurmetingen. Ned Tijdschr Geneeskd 147: 1762, 2003

  • Park JM, Terabe M, Van den Broeke LT, Donaldson DD, and Berzofsky JA: Unmasking immunosurveillance against a syngeneic colon cancer by elimination of CD4+ NKT regulatory cells and IL-13. Int J Cancer 114:80-87, 2005

  • Van den Broeke LT, Pendleton CD, Mackall C, Helman LJ and Berzofsky JA: Identification and epitope enhancement of a PAX-FKHR fusion protein breakpoint epitope in alveolar rhabdomyosarcoma cells created by a tumorigenic chromosomal translocation inducing CTL capable of lysing human tumors. Cancer Res 66: 1818-1823, 2006

  • Berzofsky JA, Van den Broeke LT, Mackall C and Helman LJ: Immunogenic peptides and methods of use for treating and preventing cancer. US Patent Appl No 12/092 449, 02 May 2008

Courses

 
Leon.png
 

Laboratory Science

P.O. Box 94
NL-4330 AB Middelburg
Office hours: by appointment
Tel. 0118-655500/ fax 0118-655508
E-mail: l.broeke@ucr.nl