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 Dr. Karine Steketee

Profile
Karine Steketee is a medical biologist, specialized in molecular genetics. She studied Biology at Leiden University. She conducted a minor graduation project, entitled Extracellular phosphorylation of adenosine by Dictyostelium discoideum, which was performed at the Department of Cell Biology of the State University Leiden under the supervision of Ir. P.H.A.M. Michielsen. Her major graduation project was done at the Department of Human Genetics under the supervision of Prof. Dr. R.R. Frants and Dr. Ir. J.A.P. Willems van Dijk. The title of the project was 'Characterization of murine lipoxygenase genes', and it was part of an atherosclerosis research project.

Her Ph.D. project, conducted at the ErasmusMC (Department of Pathology, Josephine Nefkens Institute), is entitled ‘Molecular mechanisms of the androgen receptor’, a molecular topic in the context of prostate cancer research, and is supervised by Prof. Dr. J. Trapman.

Since August 2003 she has been employed by the Christelijke Scholengemeenschap Walcheren (secondary school). In 2004 she was a lecturer at the Department of Chemistry and Aquatic Ecotechnology of the Hogeschool Zeeland. Since August 2005 she has been working as a university teacher in the Science Department at University College Roosevelt.

Academic Qualifications

  • 1991 Bachelors degree; Higher Laboratory Education (HLO): Chemistry, specialisation: Biochemistry Hogeschool Zeeland, Vlissingen,
  • 1994 Masters degree Biology:
    Medical Biology, specialisation: Molecular Genetics Leiden University, Leiden.
  • 2006 Masters degree in Education (Biology),
    Leiden University Graduate School of Education (ICLON), Leiden.
  • 2007 Ph.D. degree,
    Department of Pathology (School of Medicine), Josphine Nefkens Institute, ErasmusMC, Rotterdam

Professional Appointments

  • 1986-1987 Nurse trainee, Stichting Oosterscheldeziekenhuizen, Goes, The Netherlands.
  • 1994 Research Assistant, Department of Anthropogenetics, State University Leiden, Leiden, The Netherlands.
  • 1994-1999 Ph.D. student, Department of Pathology, Josephine Nefkens Institute, ErasmusMC, Rotterdam, The Netherlands.
  • 1999-2000 Postdoc, Department of Pathology, Josephine Nefkens Institute, ErasmusMC, Rotterdam, The Netherlands.
  • 2000-2007 Guest worker, Department of Pathology, Josephine Nefkens Institute, ErasmusMC, Rotterdam, The Netherlands.
  • 2003-present Teacher in Biology, Chemistry, Nature, Life and Technology, and General Nature Sciences, Christelijke Scholengemeenschap Walcheren, Middelburg, The Netherlands.
  • 2004 Lecturer Microbiology and Molecular Cell Biology, Departments of Chemistry and Aquatic Ecotechnology, Hogeschool Zeeland, Vlissingen, The Netherlands.
  • 2005-present University teacher in the Science Department at
  • University College Roosevelt.

Publications

  • A bioinformatics-based functional analysis shows that the specifically androgen-regulated gene SARG contains an active direct repeat androgen response element in the first intron. Karine Steketee K, Angelique C.J. Ziel-van der Made, Hetty A.G.M. van der Korput, Adriaan B. Houtsmuller and Jan Trapman (J Mol Endocrinol. 2004, 33:477-91).
  • Amino acids 3-13 and amino acids in and flanking the 23FxxLF27 motif modulate the interaction between the amino-terminal and ligand binding domain of the androgen receptor. Karine Steketee, Cor A. Berrevoets, Erik Jan Dubbink, Paul Doesburg, Remko Hersmus, Albert O. Brinkmann and Jan Trapman (Eur J Biochem. 2002 Dec;269(23):5780-11).
  • Broadened ligand responsiveness of androgen receptor mutants obtained by random amino acid substitution of H874 and mutation hot spot T877 in prostate cancer. Karine Steketee, Leon Timmerman, Angelique C.J. Ziel-van der Made, Paul Doesburg, Albert O. Brinkmann and Jan Trapman(Int J Cancer. 2002 Jul 20;100(3):309-17).
  • Mechanisms of androgen receptor activation and function.Brinkmann AO, Blok LJ, de Ruiter PE, Doesburg P, Steketee K, Berrevoets CA, Trapman J.(J Steroid Biochem Mol Biol. 1999 Apr-Jun;69(1-6):307-13 Review).
  • Functional interactions of the AF-2 activation domain core region of the human androgen receptor with the amino-terminal domain and with the transcriptional coactivator TIF2 (transcriptional intermediary factor2). Berrevoets CA, Doesburg P, Steketee K, Trapman J, Brinkmann AO.(Mol Endocrinol. 1998 Aug;12(8):1172-83).
  • Both androgen receptor and glucocorticoid receptor are able to induce prostate-specific antigen expression, but differ in their growth-stimulating properties of LNCaP cells.Cleutjens CB, Steketee K, van Eekelen CC, van der Korput JA, Brinkmann AO, Trapman J.(Endocrinology. 1997 Dec;138(12):5293-300).
  • Functional in vivo interaction between the amino-terminal, transactivation domain and the ligand binding domain of the androgen receptor. Doesburg P, Kuil CW, Berrevoets CA, Steketee K, Faber PW, Mulder E, Brinkmann AO, Trapman J.(Biochemistry. 1997 Feb 4;36(5):1052-64).
  • Genomic and cDNA cloning of a novel mouse lipoxygenase gene. Willems van Dijk K, Steketee K, Havekes L, Frants R, Hofker M.(Biochim Biophys Acta. 1995 Oct 26;1259(1):4-8)

Research Experience

  • Modification of plant gene promoters using molecular techniques, transgenesis, and qualitative analysis of transgenic plants.
  • Analysis of putative extracellular phophorylation of adenosine by Dictyostelium discoideum using HPLC and ion exchange chromatography.
  • Isolation and characterisation of the mouse 15-lipoxygenase gene using genomic library screening and standard molecular techniques.
  • Ph.D. project: 'Molecular mechanisms of androgen receptor function'(related to prostate cancer research): (1) Study of protein-protein interactions between androgen receptor subdomains using the yeast two-hybrid system, mammalian cell transfection, pull-down experiments and standard molecular techniques; (2) Isolation and characterisation of a specifically androgen regulated gene using differential display, in silico experiments, mammalian cell transfection, band shift analysis (EMSA), and chromatin immuno precipitation (ChIP); (3)Isolation of known and unknown androgen receptor mutants with broadened hormone specificity by screening a randomly mutated androgen receptor cDNA library in yeast, followed by analysis of these mutants hormone responses in mammalian cells.

Teaching Experience

  • Supervision of research projects carried out by HLO, Biology and Medical students.
  • Teaching Biology, Chemistry, Nature, Life and Technology,  and General Nature Sciences at secondary schools.
  • Giving lectures and practica in Microbiology, Molecular Biology and Molecular Cell Biology at HBO level.
  • Giving Lectures for the Introduction to Life Science and Molecular Biology and Cell Biology courses and teaching the Life Science Laboratory Course at UCR.

Courses

Karine Steketee.jpg
Life Sciences

P.O. Box 94
NL-4330 AB Middelburg
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Tel. 0118-655528 / fax 0118-655508